|
 |
| * Repair process is only used in paraffin-embedded sections(cultured cells and frozen section omit this step). |
| ** Wash with 0.02M PBS (AR0030) in Peroxidase System or 0.01M TBS (AR0031) in other systems. |
*** Stain with DAB chromogenic kit (AR1022 or AR1025) in Peroxidase System or BCIP/NBT chromogenic kit
(AR0043 or AR1023) in Alkaline Phosphatase system.
|
| **** Counterstainwith haematoxylin in Peroxidase System or nuclear fast red in Alkaline Phosphatase system. |
|
Kit Components of ECM Kit
ECM Kit (Every kit has sufficient reagents for 800 cm² of membranes ) |
Name |
Size |
| 1.Protein dry powder |
10g |
2.Affinity purified antibody
|
0.1ml (2000‐10000×) |
3.Enhanced chemiluminescent
chromogenic reagent |
Chromogenic reagent A |
5ml (20×) |
| Chromogenic reagent B |
5ml (20×) |
Kit Components of DAB Kit
DAB Chromogenic Kit (Every kit has sufficient
reagents for 800 cm² of membranes ) |
Name |
Size |
| 1. Protein dry powder |
2× 10g |
2. Concentrated antibody diluentsolution
|
20 ml (10×) |
3. Affinity purified antibody
|
0.2ml (200‐400×) |
| 4. DAB chromogenic reagent |
Chromogenic reagent A |
3ml (40×) |
| Chromogenic reagent B |
3ml (40×) |
| Chromogenic reagent C |
3ml (40×) |
|
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