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Boster provides custom antibody service to our customers worldwide, including researchers of universities, hospitals, scientific institutions and biotechnology and pharmaceutical companies. Listed below are the different custom antibody production services we offer.
Phosphospecific Antibody
* Quality without Guarantee Program
For some or all animals may fail to give a response to certain peptides, Boster does not offer guarantee regarding our custom antibody service. Do not accept this program? Please go "Quality Guarantee Program". |
| Phase |
Time & Price |
Ⅰ. Peptide synthesis
* Phosphospecific peptide synthesis (up to 15 amino acids): 15mg, purity >90%
§Phosphospecific epitope affinity purification: 5mg
§Antibody production: 5mg
§Delivery to customer: 5mg
* Non-phosphospecific peptide synthesis (up to 15 amino acids): 10mg, purity >80%
§Affinity depletion: 5mg
§Delivery to customer: 5mg |
2 Weeks |
Ⅱ.Conjugation (Determined by customers)
* KLH conjugation
* BSA conjugation
* Hemocyanin conjugation |
1 week |
Ⅲ.Immunization and anti-serum production
* Immunization : 2 rabbits
* Pre-immune sample of serum 5ml/per rabbit and anti-serum (100ml) |
8 weeks |
Ⅳ.ELISA test
* Phosphospecific and non-phosphospecific peptide ELISA titration of antibody for all bleeds |
1 week |
Ⅴ.Purification and antibody production
* Affinity purification of anti-serum
* Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column
* WB or WB+IHCP test, but do not guarantee the quality
* Delivery to customer: 5mg purified phosphospecific antibody |
2 weeks |
| Total |
14 weeks ($2,000) | |
| *Quality Guarantee Program |
| Phase |
Time & Price |
Ⅴ.Purification and antibody production
* Affinity purification of anti-serum
* Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column
* WB or WB+IHCP test with quality guarantee
* Delivery to customer: 2mg purified phosphospecific antibody |
2-3 weeks ($4,000) |
Ⅴ.Purification and antibody production
* Affinity purification of anti-serum
* Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column
* WB or WB+IHCP test with quality guarantee
* Delivery to customer: 5mg purified phosphospecific antibody |
2-3 weeks ($7,000) | |
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Note:
1) Boster will provide custom antibody service in the shortest possible time. Delays may be incurred for some inexpectant reasons, eg. the antigen is poorly immunogenic.
2) Usually, Boster will use our own specimen to test the custom antibody, but the peculiar or rare species (eg. Animal model, culture cell) should be provided by customers.
3) If your customized phosphospecific antibody development just need a part of the above protocols, please contact us for a quotation.
4) If 5mg antibody is not enough, please contact us for a quotation.
5) For the successful polypeptide antibody, if necessary, Boster can develop and manufacture monoclonal antibody with this polypeptide for you. | |
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